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After 2 h incubation, cells were washed twice with cold PBS and were extracted with 80% ice-cold methanol at 2 10 6 cells/mL

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This disorder is associated with severe congenital microcephaly, severe 2-oxoglutaric aciduria (-ketoglutaric aciduria), and death within the first year

10.1074/jbc.270.30.17641 271 SetoguchiS.MohriM.ShimokawaH.TakeshitaA

This immediate source of glutathione is considered a first line defence against oxidative stress-induced damage 12